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Antibodies

Abi1 Antibody

Product Sizes
25 ul
600-401-GT5S-25UL
About this Product
SKU:
600-401-GT5S
Additional Names:
Abi1|rabbit anti-Abi1 Antibody, Abi-1, Abi 1, Abl Interactor 1, Eps8 SH3 Domain-Binding Protein, Abl-Binding Protein, Abelson Interactor 1, E3B1, Spectrin SH3 Domain Binding Protein 1, Nap1-binding protein, SSH3BP1
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.07 mg/ml
Extra Details:
Anti-Abi1 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Abi1 gene has been suggested to have a role in inhibiting cell growth and encodes an adaptor protein in the Abelson-interactor family. The protein is involved in signal transduction, actin regulation and cytoskeletal remodeling. Studies suggest that Abi1 is a member of the WAVE complex and a dysfunctional complex may allow for the progression of prostate cancer. Anti-Abi1 is ideal for researchers interested in melanoma, colon and breast cancer and other cancer antibody research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to near c-terminal of the mouse Abi-1 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-Abi1 is directed against mouse Abi1. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest cross reactivity with this protein in human and rat based on 100% homology for the immunogen sequence. Reactivity against homologues from other sources is not known.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit anti-Abi1 antibody. Marker: BioRad Precision Plus Dual Color Standard. Lane 1: Purified recombinant His-tagged Abi1 Isoform 2. Lane 2: Purified recombinant His-tagged Abi1 Isoform 3. Load: 2 uL per lane. Primary antibody: Abi1 antibody at 1:1,000 for 2hrs at RT. Secondary antibody: HRP conjugated goat-A Alpha-rabbit at 1:10,000 for 60 min at RT. Blocking Buffer: 5% non-fat milk in TBS-t overnight at 4°C. Predicted/Observed size: 65 kDa for Abi1.
Western Blot of Rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: Abi1 recombinant protein. Load: 50 ng per lane. Primary antibody: Abi1 antibody at 1:1,000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Blocking Buffer: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.
Western Blot of Rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: MEF3 KO. Lane 2: MEF3 WT. Lane 3: Mouse brain (p/n W10-000-T004). Lane 4: Rat brain (p/n W12-000-T077). Lane 5: HCT-116 WCL (p/n W09-001-GM4). Lane 6: HeLa WCL (p/n W09-000-364). Lane 7: NIH/3T3 WCL (p/n W10-000-358). Load: 35 ug per lane. Primary antibody: Abi1 antibody at 1:1,000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Blocking Buffer: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.