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Antibodies

BrdU Antibody

Product Sizes
100 ug
600-401-C29-100UG
About this Product
SKU:
600-401-C29
Additional Names:
rabbit anti-BrdU Antibody, bromodeoxyuridine, 5-bromo-2'-deoxyuridine
Application:
Detection/Quantitation, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.1 mg/ml
Extra Details:
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH.
Isotype:
IgG
Physical State:
Liquid
Shipping Conditions:
Dry Ice
Specificity:
Anti-BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage Conditions:
2-8[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of Rabbit anti-BrdU antibody. Tissue: 293T cells transfected expressing GFP-MCM9 L. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Anti-rabbit ATTO550 secondary antibody at 1:10,000 for 45 min at RT. Localization: BrdU is nuclear. Staining: BrdU in merged image shows with green and purple fluorescent signal with DAPI nuclear counterstain.
Immunofluorescence microscopy images of paraformaldehyde-fixed, paraffin-embedded pancreas sections stained with antibodies against BrdU (red or pink) and counterstained with DAPI (blue) and imaged with a 40x objective. DAPI stained nuclei (blue) indicate non-dividing cells, immunostained red and pink nuclei indicate actively dividing pancreatic B Beta-cells. The antibodies were diluted to 2.7 ug/ml. and incubated with tissue sections overnight at 4 degrees. Donkey anti-rabbit secondary antibody was diluted 1:2500.
Bromodeoxyuridine (BrdU) chemical structure representation.
SOX2 represses proliferation in the developing spinal cord and stomach.(A) Percentage of cells expressing high or low levels of SOX2 labelled by a one hour pulse of BrdU in the E11.5 mouse spinal cord. Dotted lines in insets surround area of greatest BrdU incorporation. (B) Average background normalized SOX2 expression level and percentage of cells labelled by a one hour pulse of BrdU in the E11.5, E13.5 and E15.5 anterior and posterior stomach. (C) Percentage of electroporated cells in the chick spinal cord labelled by a 30 minute pulse of BrdU following misexpression of GFP, SOX2 or dnSOXB1. (D) Percentage of electroporated cells in E13.5 stomach explants labelled by a 30 minute pulse of BrdU following overexpression of GFP, SOX2 or dnSOXB1. All error bars represent standard deviations between experiments and p-values are calculated with two sided, unpaired t-tests (* = p<0.05, ** = p<0.01, *** = p<0.001). Figure provided by CiteAb. Source: PLoS Genet, PMID: 29432416.