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Antibodies

CASZ1 Antibody

Product Sizes
100 ug
600-401-B62-100UG
About this Product
SKU:
600-401-B62
Additional Names:
CASZ1|rabbit anti-CASZ1 Antibody, CASZ1, Zinc finger protein castor homolog 1, Castor-related protein, Zinc finger protein 693, CST, SRG, ZNF693
Application:
ChIP, Detection/Quantitation, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.07 mg/ml
Extra Details:
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCASZ5 and hCASZ11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in fresh lysates.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human CASZ1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry results of Rabbit Anti-hCASZ1 Antibody. Tissue: NB patient tumor. A. Score 0- a rare positive nuclei. B. Score 1- (1-10% positive) equivocal/uninterpretable. C. Score 2- (10-50% positive) weak positive. D. Score 3- (>50% positive) strong positive. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue). Localization: Nuclear.
Immunohistochemistry results of Rabbit Anti-hCasz1 Antibody. Tissue: NB patient tumor. A. CASZ1 localized exclusively in the cytoplasm. B. CASZ1 localized in the cytoplasm and nucleus. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue).
Immunofluorescence results of Endogenous CASZ1. Cells: BE2 cells. With or without Pre-Incubation of Anti-CASZ1 Antibody with CASZ1 Peptide. Staining: Rabbit Anti-CASZ1 Antibody. Chromatin counter stain: DAPI.
Immunofluorescence results of Rabbit Anti-CASZ1 Antibody. Tissue: Mouse Xenograft tumor of human NB cell line transfected with or without tetracycline inducible CASZ1 (NGPtetCASZ1a). Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI.
Immunofluorescence of Rabbit anti-CASZ1 Antibody. Tissue: adult murine ocular tissue. Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI. Localization: nucleus in lens epithelia but primarily localizes in the cytoplasm in photoreceptor cells.