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Antibodies

CTRP1 Antibody

Product Sizes
100 ug
600-401-AP5-100UG
About this Product
SKU:
600-401-AP5
Additional Names:
C1QTNF1|CTRP1 Antibody, GIP, CTRP1, ZSIG37, UNQ310/PRO353, Complement C1q tumor necrosis factor-related protein 1, G protein-coupled receptor-interacting protein, GIP
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. In obese (ob/ob) mice, RT-PCR analysis showed that mCTRP1 transcripts are seen at substantially higher levels in adipose tissues compared to those of normal mice.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CTRP1 (NT) antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the amino-terminus of human CTRP1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-CTRP1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of CTRP1 antibody Lane A: CTRP1 antibody at 1 ug/mL. Lane B: CTRP1 antibody at 2 ug/mL. Load: in human kidney cell lysate 35 ug per lane. Primary antibody: CTRP1 antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 32 kDa, 30 kDa for CTRP1. Other band(s): CTRP1 splice variants and isoforms.
Immunofluorescence Microscopy of CTRP1 antibody. Cell Type: human kidney cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CTRP1 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CTRP1 is secreted. Staining: CTRP1 as green fluorescent signal.
Immunohistochemistry of CTRP1 antibody. Tissue: human kidney tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: CTRP1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CTRP1 is secreted. Staining: CTRP1 is stained with hematoxylin purple nuclear counterstain.