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Antibodies

CARMA1 Antibody

Product Sizes
100 ug
600-401-AB8-100UG
About this Product
SKU:
600-401-AB8
Additional Names:
CARD11|CARMA1 Antibody, PPBL, BENTA, BIMP3, IMD11, CARMA1, Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1, Carma 1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IkK complex, thereby regulating activation of the NF-kB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CARMA1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-CARMA1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with CARMA1 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of CARMA1 antibody. Lane A: mouse thymus cell lysate at 0.5 ug/ml. Lane B: mouse thymus cell lysate at 1 ug/ml. Lane C: mouse thymus cell lysate at 2 ug/ml. Load: 35 ug per lane. Primary antibody: CARMA1 antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 133 kDa, 130 kDa for CARMA1. Other band(s): CARMA1 splice variants and isoforms.
Immunofluorescence Microscopy of CARMA1 antibody. Cell Type: mouse spleen cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CARMA1 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CARMA1 is located in the cytplasm and membrane raft. Staining: CARMA1 as red fluorescent signal.
Immunohistochemistry of CARMA1 antibody. Tissue: mouse thymus. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: CARMA1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CARMA1 is located in the cytoplasm and membrane raft. Staining: CARMA1 is stained with hematoxylin purple nuclear counterstain.