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Antibodies

ABCB1 Antibody

Product Sizes
25 ul
600-401-967S-25UL
About this Product
SKU:
600-401-967S
Additional Names:
ABCB1|rabbit anti-ABCB1 Antibody, ABCB 1, ABCB-1, ATP binding cassette sub family B MDR/TAP member 1 antibody, CD243 antibody, CD243 antigen antibody, CLCS antibody, GP170 antibody, MDR 1 antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). ATP-Binding Cassette Sub-Family B Member 1 (ABCB1, also named P-glycoprotein) is a plasma membrane-associated multidrug transporter that utilizes the energy of ATP hydrolysis to pump toxic xenobiotics out of cells. Unique features of ABCB1 are its very broad substrate specificity and its basal ATPase activity in the absence of transport substrates. Human ABCB1 plays an important role in absorption, distribution, metabolism, excretion and toxicity of pharmacologically relevant drugs. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anti-cancer drugs. This protein also functions as a transporter across the blood-brain barrier.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 255-280 of human ABCB1 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This affinity-purified antibody is directed against human ABCB1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with ABCB1 protein from human, monkey, dog, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western blot using Rockland's affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10,000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
IHC using Rockland ABCB1 Antibody at 20 ug/ml against FFPE human kidney. Moderate to strong cytoplasmic and membranous staining seen in this example. Similar staining also observed in other tissues including liver, kidney, and small intestine. The antibody showed minimal background staining and worked well in formalin-fixed tissues. Image provided courtesy of Lifespan Biosciences.