Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Cas5 Antibody

Product Sizes
100 ug
600-401-948-100UG
About this Product
SKU:
600-401-948
Additional Names:
hCAS5|rabbit anti-Cas5 antibody, Castor homolog 1 zinc finger protein, CST, SRG, ZNF693, CASZ1, Cas-5, Cas 5
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.3 mg/ml
Extra Details:
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. hCas5 (Castor) is the human homolog of a Drosophila gene that regulates neurogenesis. In human neuro-blastoma tumors where a loss of heterozygosity of CASZ1 is noted, hCas functions to suppress the tumorigenicity of cells. hCas5 is a developmentally regulated transcription factor found in neural and muscle cells. hCas5 is a Zn finger transcription factor that is predominantly found in the nucleus. This antibody is designed to facilitate the functional study of this protein and its role in developmental regulation and expression in human tumor tissues.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This affinity-purified antibody is directed against human Cas5 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Cas5 from human, mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western blot using Rockland's affinity purified anti-hCas5 antibody. Lane 1: AS cell lysate transfected with empty vector. Lane 2: AS cell lysate transfected with hCas5 (predicted MW 125kDa). Lane 3: BE2 cell lysate that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4: human muscle tissue lysate that shows high levels of hCas mRNA. Shows detection of a band ~125kDa in size corresponding to hCas5 in lane 2 (arrowhead). Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.