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Antibodies

BACH1 Antibody

Product Sizes
100 ug
600-401-458-100UG
About this Product
SKU:
600-401-458
Additional Names:
BRIP1|rabbit anti-BACH1 antibody, BACH-1, BACH 1, Fanconi anemia group J protein, FACJ, BRAC 1 Associated C Terminal Helicase 1 antibody, BRCA 1 Interacting Protein 1 antibody, BRCA1 binding helicase like protein BACH1 antibody, BRCA1 interacting protein C terminal helicase 1 antibody, BRIP 1 antibody, ATP-dependent RNA helicase BRIP1, FANCJ, FANC-J, FANC J
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 92-104 of isoform 1 of human BACH1 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human BACH1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with BACH1 protein from human (100% homology) and chimpanzee (92% homology). Expect reactivity with isoform 1 and isoform 2 of BACH1. Reactivity against BACH1 homologues from rat
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western blot using Rockland's Affinity Purified anti-BACH1 antibody shows detection of a band at ~105 kDa (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (p/n W09-000-365). Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.