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Antibodies

GST Antibody Dylight[TM] 649 Conjugated

Product Sizes
100 ug
600-143-200-100UG
About this Product
SKU:
600-143-200
Additional Names:
goat anti-GST antibody DyLight™ 649 conjugation, DyLight™ 649 conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody
Application:
FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 649
Concentration:
1 mg/ml
Extra Details:
Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
The immunogen is full length GST isolated from Schistosoma japonicum.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST (Schistosoma japonicum) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Glutathione-S-Transferase (GST).
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Tagged Antibodies
Immunofluorescence using Anti-GST DyLight™649 Conjugated Antibody. FYVE and PX PtdIns3P-binding domains detect both endosomal and autophagosomal pools of PtdIns3P. HeLa cells were transiently transfected with GFP-2xFYVE or GFP-P40PX expression vectors or were mock transfected. Mock-transfected cells were incubated after fixation with primary antibodies or GST-2xFYVE or GST-mut2xFYVE and then with appropriate secondary antibodies. Representative confocal microscopy images of tagged PtdIns3P-binding domain (green), LC3 (blue), EEA1 (magenta), and DAPI (orange) fluorescence. Blue and magenta arrowheads indicate codistribution/colocalization of tagged PtdIns3P-binding domains with LC3 or EEA1, respectively. Scale bar: 10 um. Fig 1. PMID: 28813193.
Properties of DyLight™ Conjugates.
DyLight™ dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.