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Antibodies

Collagen Type IV Antibody

Product Sizes
100 ug
600-101-MN4-100UG
About this Product
SKU:
600-101-MN4
Additional Names:
Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, alpha unit Collagen IV Type IV Collagen, Col4, Collagen alpha-1(IV) chain|COL4A1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.18 mg/ml
Extra Details:
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagen Type IV proteins are integral components of basement membranes. This type of collagen is found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Anti-Collagen Type IV Antibody is ideal for researchers involved in extracellular matrix proteins and nephritis.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-Collagen IV antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 240-265 of mouse Collagen IV.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against mouse Collagen IV. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with mouse and 78.6% identity with human.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence of Goat Anti-Collagen Type IV Antibody. Cells: NIH/3T3 cells. Fixative: 4%PFA. Permeabilization: None. Primary Antibody: Anti-Collagen Type IV at 15ug/mL overnight at 2-8°C. Counterstain: Donkey Anti-Goat IgG Dylight™488 (p/n 605-741-125) at 5ug/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-Collagen Tye IV + Dylight™488. (C) Merge A+B. (D) secondary only. Expected localization: Extracellular, ER.
Immunohistochemistry of Goat Anti-Collagen Type IV Antibody. Tissue: Mouse Placenta. Fixative: None. Antigen Retrieval: HIER using Citrate Buffer for 20min. Primary Antibody: Anti-Collagen Type IV at 1:100 for 30 min at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP at 4uL/mL for 20 in at RT. Counterstain: Hematoxylin. Substrate: DAB. Results: shows intense staining of trophoblastic cells and endothelial cells.
Immunohistochemistry of Goat Anti-Collagen Type IV Antibody. Tissue: Mouse Testes. Fixative: None. Antigen Retrieval: HIER using Citrate Buffer for 20min. Primary Antibody: Anti-Collagen Type IV at 1:100 for 30 min at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP at 4uL/mL for 20 in at RT. Counterstain: Hematoxylin. Substrate: DAB. Results: shows moderate staining of Leydig cells and no significant staining of the seminiferous tubules.