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Antibodies

Brn3b Antibody

Product Sizes
100 ug
600-101-MJ0-100UG
About this Product
SKU:
600-101-MJ0
Additional Names:
Goat Anti-Brn3b Antibody, Goat Anti-Pou4f2 Antibody, POU domain, class 4, transcription factor 2, Brain-specific homeobox/POU domain protein 3B, Brn-3B, Brain-3B, Brn-3.2|Pou4f2
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.05 mg/ml
Extra Details:
Brn3b (Brain-Specific Homeobox/POU Domain Protein 3B) or POU4F2 (POU Class 4 Homeobox 2) is a DNA-binding transcriptional regulator and coregulator that recognizes and binds to the consensus octamer binding site 5-AT[A/T]A[T/A]T[A/T]A-3 in promoter of target genes. Brn3b plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It cooperates with the transcription factor ISL1 to achieve RGC fate specification in the developing retina. And plays a role in RGC axon formation and guidance by regulating gene expression of specific target genes. Brn3b plays a role in TNFSF11-mediated terminal osteoclast differentiation. It binds chromatin at promoter region of target genes. It acts either as a transcriptional activator or repressor. It plays a role in the regulation of breast cancer cell growth by promoting transcription activation as well as repression of specific target genes. It is involved in tumor breast progression and invasion. Brn3b also plays a role either as a transcriptional coactivator or corepressor. Anti-Brn3b Antibody is useful for researchers interested in breast cancer research, transcription factor activity, and DNA binding.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-Brn3b antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse Pou4f2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against mouse Brn3b. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat and 91.7% to human.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Goat Anti-Brn3b Antibody. Lane 1: Opal Prestained Molecular weight ladder (p/n MB-210-0500). Lane 2: Rat pup PND2-6 - minimal cortex brain lysate (35ug) [+]. Lane 3: HEK293T whole cell lysate (35ug) [-]. Primary Antibody: Anti-Brn3b at 1:1000 overnight at 2-8°C. Secondary Antibody: Donkey anti-Goat IgG HRP (p/n 605-703-125) at 1:40,000 for 30 minutes at RT. Block: BlockOut Buffer (p/n MB-073). Exposure: 12 sec. Predicted MW: ~43kDa (rat), ~35, ~43kDa (mouse). Observed MW: ~47kDa.
Immunofluorescence of Goat Anti-Brn3b Antibody. Cell Line: Post-natal rat pup (PND1) heterogeneous brain cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Brn3b at 5ug/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 15ug/mL for 1 hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Brn3b + secondary. (C). Merge A+B. (D). secondary only. Expected localization: Nuclear, nuclear speckle.
Immunohistochemistry of Goat Anti-Brn3b Antibody. Tissue: Mouse Testis. Fixative: none. Antigen Retrieval: HIER using Citrate Buffer for 20 minutes. Primary Antibody: Anti-Brn3b at 1:100 for 30 minutes at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP 4ul/mL for 20 minutes at RT. Counterstain: Hematoxylin. Substrate: DAB. Analysis: BRN3B staining of human testes shows intense punctate intranuclear staining of Sertoli cell of the seminiferous tubules which is the expected staining pattern.
Immunofluorescence of Goat Anti-Brn3b Antibody. Tissue: Mouse Retina Tissue. Retrieval: 10mM Sodium Citrate pH6 retrieval and 10mM Tris-base 1mM EDTA 0.05% Tween 20 pH9. Permeabilization: 0.5% Triton X-100 per 15 mins. Primary Antibody: Anti-Brn3b at 1:200 overnight at 2-8°C. Incubate secondary antibody 2 hrs. Incubate HOECSHT 10 mins. Fluoro-G mounting. This antibody works with or without retrieval. Courtesy of Viviane Valenca at University of Utah.
Immunofluorescence of Goat Anti-Brn3b Antibody. Tissue: Mouse Retina Tissue. Permeabilization: 0.5%Triton X-100 per 45mins. Primary Antibody: Anti-Brn3b at 1:200 for 3 days at 2-8°C. Incubate secondary antibody for 2hrs. Incubate HOECSHT for 30 mins. Fluoro-G mounting. Courtesy of Viviane Valenca at University of Utah.