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Antibodies

BACE Antibody

Product Sizes
100 ug
200-401-Z18-100UG
About this Product
SKU:
200-401-Z18
Additional Names:
BACE Antibody, ASP2, BACE, HSPC104, KIAA1149, Beta-secretase 1, Aspartyl protease 2, ASP2|BACE1
Application:
Detection/Quantitation, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-BACE antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 17 amino acids at the C-terminus of human BACE. The immunogen is located within the last 50 amino acids of BACE.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-BACE Antibody is affinity chromatography purified via peptide column. Cross reactivity with BACE from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunocytochemistry of BACE antibody. Cells: 3T3 cells. Fixation: formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary antibody: BACE antibody at 10 ug/mL overnight at 2-8°C. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody at 1:250 for 45 min at RT. Localization: BACE is localized in the cell membrane, endoplasmic reticulum, Golgi apparatus, endosome, and the cell surface. Counter stained with Hematoxylin.
Western Blot of BACE antibody. Lane 1: Human brain tissue lysate. Lane 2: Human brain tissue lysate with the presence of blocking peptide. Lane3: Mouse 3T3 cell lysate. Load: 35 ug per lane. Primary antibody: BACE antibody at 1 ug/mL for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55.7 kDa, 76 kDa for BACE. Other band(s): BACE splice variants and isoforms.
Immunofluorescence of Rabbit Anti-BACE Antibody. Cells: NIH/3T3 Cells. Fixation: 4% Paraformaldehyde. Primary Antibody: Anti-BACE at 20ug/mL. Secondary Antibody: Goat anti-Rabbit at 1:500. Staining: DAPI. Cytosol and membrane staining.
Immunofluorescence of Anti-BACE Antibody. Immunolabeling patterns of BACE1 expression and the lenti-siRNA distribution. Sections from APP transgenic mice treated with the eGFP-tagged lenti siRNA vectors (green) were co-immunolabeled with an antibody against BACE1 (red) and imaged with the LSCM. All sections are from the hippocampus of treated mice. (a-c) Lenti-siBACE1-6-treated mice. Areas within the hippocampus expressing the eGFP-tagged vector have reduced BACE1 immunolabeling. (d-f) Mice treated with the eGFP-tagged control lenti-siGlut4 show unchanged expression of BACE1 in the hippocampus. (g-i) Mice treated with a saline vehicle show unchanged expression of BACE1 in the hippocampus. (Singer et al., 2005).
Immunofluorescence of Anti-BACE Antibody. Decreased BACE1 expression in DRG following siRNA3 transfection. DRG neurons were transfected with 1 ug siRNA3 plasmid and incubated for 48 hours in 37°C. DRG neurons were stained for BACE1 using the Anti-BACE antibody. (a,b) Neurons transfected with the control plasmid pSUPER-EGFP (green) did not display any changes in BACE1 expression (red). (c,d) DRG neurons transfected with siRNA3 displayed reduced BACE1 expression in the axon. (Hyun, 2007).