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Antibodies

Carbonic Anhydrase II Antibody

Product Sizes
100 ug
200-401-136-0100-100UG
About this Product
SKU:
200-401-136-0100
Additional Names:
CA2|rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.1 mg/ml
Extra Details:
Carbonic Anhydrase II Antibody detects Carbonic Anhydrase II. Carbonic Anhydrase II is essential for bone resorption and osteoclast differentiation by similarity. It can hydrate cyanamide to urea and is involved in the regulation of fluid secretion into the anterior chamber of the eye. Anti-Carbonic Anhydrase II Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Carbonic Anhydrase II Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Human, Mouse
Shipping Conditions:
Ambient
Specificity:
Anti-Carbonic Anhydrase II Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially puri
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.
Western Blot of Rabbit Anti-Carbonic Anhydrase II primary antibody. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Blocking: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.