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Antibodies

Ovalbumin Antibody

Product Sizes
25 ul
200-401-033S-25UL
About this Product
SKU:
200-401-033S
Additional Names:
rabbit anti-Ovalbumin Antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White|SERPINB14
Application:
Bioactivity, ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Anti-Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 55-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins, it does not undergo a conformational change upon proteolytic cleavage and is not a serine protease inhibitor. The function of ovalbumin is still unknown, although it is presumed to be a storage protein for tissue-specific, steroid hormone-induced gene expression. Ovalbumin is a key protein for biochemical investigations and processes and can be used as a control, blocking reagent, or antibody in assays such as ELISA, SDS-PAGE, IHC, Western Blot, and size exclusion chromatography.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Ovalbumin [Hen Egg White]
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Avian
Shipping Conditions:
Dry Ice
Specificity:
Rabbit Anti-Ovalbumin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit anti-OVALBUMIN (Hen Egg White) antibody. Lane 1: Reduced Ovalbumin. Load: 50 ng. Primary antibody: Ovalbumin antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin.
Western Blot of Rabbit anti-Ovalbumin antibody. Lane 1: Ovalbumin protein - Reduced [~1 ug]. Lane 2: Ovalbumin protein - Reduced [0.25 ug]. Lane 3: Ovalbumin protein - Non-reduced [~1 ug]. Lane 4: Ovalbumin protein - Non-reduced [0.25 ug]. Primary antibody: Ovalbumin antibody at 1:5000 for overnight at 4°C. Secondary antibody: Atto 425 conjugated goat anti-rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block: MB-070 buffer overnight at 4°C. Predicted/Observed size: 42.9 kDa, ~36 kDa for ovalbumin. Other band(s): none.