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Antibodies

Alpha-1-Anti-Trypsin Antibody

Product Sizes
25 ul
200-101-147S-25UL
About this Product
SKU:
200-101-147S
Additional Names:
goat anti-Alpha-1-Trypsin Antibody, A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibody|SERPINA1
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Alpha-1-Antitrypsin (AAT) is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. It irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT is reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
a1-Anti-Trypsin [Human Plasma]
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-ALPHA-1-ANTI-TRYPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified a1-Anti
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Goat anti-Alpha-1-Anti-Trypsin antibody. Lane 1: Albumin depleted human serum. Load: ~10 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody (p/n 605-4302) at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none
Western Blot of Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 at 30 min RT.
Western Blot of Goat anti Alpha-1 anti-Trypsin antibody. Lane 1: Alpha-1 anti-Trypsin under reducing conditions. Load: 1 ug per lane. Primary antibody: Alpha-1 anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: DyLight 488 conjugated Donkey anti-goat secondary antibody at 1:10,000 for 45 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 55kDa for A Alpha-1 Anti-Trypsin. Other band(s): none.
Western Blot of goat anti-Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4°C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight™ 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight™ 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: Blocking Buffer for Fluorescent Western Blotting - MB-070 overnight at 4°C