Goat TrueBlot[R]: Anti-Goat IgG HRP
Product Sizes
50 ul
18-8814-31-50UL
About this Product
- SKU:
- 18-8814-31
- Additional Names:
- Anti-Goat IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
- Application:
- Immunoprecipitation, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- eB270
- Clonality:
- Monoclonal
- Conjugate:
- HRP
- Concentration:
- 1 mg/ml
- Extra Details:
- Goat IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Goat IgG monoclonal secondary antibody. Goat IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Goat IgG TrueBlot®, simply substitute the conventional HRP Anti-Goat IgG blotting reagent with Goat IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Goat IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of Goat IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Goat IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Mouse
- Isotype:
- IgG
- Physical State:
- Liquid
- Reactivities:
- Goat
- Shipping Conditions:
- Blue Ice
- Specificity:
- Goat TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum. Reactivity is observed against native Goat IgG by both Western blot and ELISA.
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Secondary Antibodies
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=18-8814-31
