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Proteins and Peptides

Leptin pegylated antagonist (mutant L39A/D40A/F41A)

Product Sizes
50 ug
£404.71
500-066-50UG
100 ug
£674.12
500-066-100UG
About this Product
SKU:
500-066
Additional Names:
Lep; ob; obese
translate.label.attr.clone:
(#14G5)
Extra Details:
Mono-pegylated (with 20 kDa PEG)recombinant ovine leptin antagonist is one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Ovine leptin was initially mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques according to Niv-Spector et al (2005) Biochemical Journal, 291, 221-230 and its pegylation was similar to that of mouse leptin antagonist is described in Elinav et al. Endocrinology (2009)150, 3083-3091.
Formulation:
lyophilized
Host:
Rabbit
Molecular Weight:
35.6 kDa
Purity:
> 95.0% as determined by Gel filtration and SDS-PAGE gel.
Reactivities:
Sheep
Sequence:
AVPIRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGAAAIPGLHPLLSLSKMDQTLAIYQQILASLPSRNVIQISNDLENLRDLLHLLAASKSCPLPQVRALESLESLGVVLEASLYSTEVVALSRLQGSLQDMLRQLDLSPG
Shipping Conditions:
Ambient
Storage Conditions:
Room Temperature
Supplier:
ReliaTech
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins