Sonic Hedgehog
Product Sizes
100 ug
£674.12
103-M476-100UG
About this Product
- SKU:
- 103-M476
- Additional Names:
- Shh; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
- Application:
- Block/Inhibit/Neutralise, IHC-Frozen, Western Blot
- Buffer:
- PBS
- translate.label.attr.clone:
- 6K12
- Clonality:
- Monoclonal
- Extra Details:
- Human Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage. Although the binding affinity to their receptors is not changed, lipid-modified Shh-N proteins are more potent than the unmodified proteins in cell-based assays. Other hydrophobic modifications to unmodified Shh-N, including the substitution of the N-terminal cysteine residue with two hydrophobic isoleucine residues, can also increase Shh-N potency.
- Formulation:
- lyophilized
- Host:
- Rat
- Immunogen:
- recombinant mouse Shh (N-terminal) fragment
- Isotype:
- IgG2
- Purification:
- Protein G Purified
- Reactivities:
- Mouse
- Shipping Conditions:
- Ambient
- Storage Conditions:
- -20[o]C lyophilized. Avoid freeze/thaw cycles.
- Supplier:
- ReliaTech
- Type:
- Antibody: Monoclonal Antibody