HGF-R
Product Sizes
100 ug
£674.12
101-M450-100UG
About this Product
- SKU:
- 101-M450
- Additional Names:
- MET; HGFR; AUTS9; RCCP2; c-Met
- Application:
- Western Blot
- Buffer:
- PBS
- translate.label.attr.clone:
- 5B36
- Clonality:
- Monoclonal
- Extra Details:
- HGF R, also known as Met (from N-methylN' nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes co-translational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular A Alpha chain and a 145 kDa transmembrane B Beta chain. The extracellular domain (ECD) contains a seven bladed B Beta-propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain. Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the A Alpha and B Beta chains. The sema domain, which is formed by both the A Alpha and B Beta chains of HGF R, mediates both ligand binding and receptor dimerization. Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules. HGF stimulation induces HGF R downregulation via internalization and proteasomedependent degradation. In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin A Alpha6/B Beta4, Plexins B1, 2, 3, and MSP R/Ron. Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects. Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion. Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells. Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers. Within the ECD, human HGF R shares 86-88% amino acid sequence identity with canine, mouse, and rat HGF R.
- Formulation:
- lyophilized
- Host:
- Mouse
- Immunogen:
- recombinant human HGFR EC domain
- Isotype:
- IgG1
- Purification:
- Protein G Purified
- Reactivities:
- Human
- Shipping Conditions:
- Ambient
- Storage Conditions:
- -20[o]C lyophilized. Avoid freeze/thaw cycles.
- Supplier:
- ReliaTech
- Type:
- Antibody: Monoclonal Antibody