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Antibodies

anti-Perilipin 2 (N-terminus), guinea pig polyclonal antibody

Product Sizes
100 ul
£384.00
GP47-100UL
About this Product
SKU:
GP47
Additional Names:
Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Adipophilin / ADRP / PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (Heid et al. 1998; Targett-Adams et al. 2003); inducible by etomoxir. Enhanced expression of Adipophilin / ADRP / PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (Hope et al. 2002). Polypeptide reacting: Adipophilin / ADRP / PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72. Tissue localization: Adipophilin / PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Reactivity on cultured cell lines: PLC. Targett-Adams, P. et al. Live Cell Analysis and Targeting of the Lipid Droplet-binding Adipocyte Differentiation-related Protein. J. Biol. Chem. 278, 15998-16007 (2003). Hope, R. G., Murphy, D. J. & McLauchlan, J. The Domains Required to Direct Core Proteins of Hepatitis C Virus and GB Virus-B to Lipid Droplets Share Common Features with Plant Oleosin Proteins. J. Biol. Chem. 277, 4261-4270 (2002). Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309-21 (1998).
Formulation:
Contains 0.09% sodium azide and 0.5% BSA
Host:
Guinea Pig
Immunogen:
Synthetic peptide (N-terminal aa 6-27 of human adipophilin / PLIN2)
Purification:
Stabilized antiserum
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:GP47
Western blot analysis of HEK lysate with anti-Perilipin 2 antibody (Cat. No. GP47). Western blot analysis was performed on 5 ug of HEK lysate either untreated or transfected causing overexpression of Perilipin 2. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibodies anti-Perilipin 2 (Cat. No. GP47) and anti-GAPDH were diluted in blocking buffer (1:500 Anti-Perilipin
1:1
500 anti-GAPDH) and incubated at 4°C over-night. The secondary antibodies donkey anti-guinea pig A647 and goat anti-mouse A488 were also diluted in blocking buffer (both 1:300) and incubated for 1 h at RT. The bands were visualized by fluorescent detection.
Immunofluorescence analysis of HepG2 cells with anti-Perilipin 2 antibody (Cat. No. GP47). HepG2 cells were analysed either untreated or treated with 400 uM oleic acide over-night to incorporate lipid droplets. Fixation was performed using 3% paraformaldehyde for 15 min at RT. Cells were blocked with 5% BSA in PBST (PBS + 0.1% Tween 20) for 1 h at RT and permeabilized with 0.3% Triton-x 100 in PBS for 10 min at RT. The primary antibody anti-Perilipin 2 guinea pig polyclonal (Cat. No. GP47) was 1:100 diluted in blocking buffer and incubated over-night at 4°C. The secondary antibody donkey anti-guinea pig AF647 was also diluted in blocking buffer (antibody concentration 3.75 ug/ml) and incubated for 30 min at 37°C and 30 min at RT. DNA was stained with DAPI in blue.