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Antibodies

anti-Perilipin 5 (N-terminus), guinea pig polyclonal antibody

Product Sizes
100 ul
£354.00
GP44-100UL
About this Product
SKU:
GP44
Additional Names:
Perilipin-5, Lipid storage droplet protein 5, PLIN5, LSDP5, OXPAT, PAT-1
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Polypeptide reacting: MLDP = myocardial lipid droplet protein (also described as OXPAT/PAT-1 and perilipin 5 or PLIN5), MW 50,844 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE). MLDP / PLIN5 pertains to the PLIN/PAT-family proteins, covering the surface of cytoplasmic lipid droplets and sharing a homologuous domain called PAT. Additional PLIN/PAT proteins include adipophilin (ADRP, PLIN2), perilipin (PLIN1), and TIP47 (PLIN3) which are expressed in differentiation-related stages of lipid metabolism. No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism. Tissue localization: MLDP/PLIN5 is positively detected in heart and skeletal muscle. Reactivity on cultured cell lines: HaCat (human), SV80 (human).
Formulation:
Contains 0.09% sodium azide and 0.5% BSA
Host:
Guinea Pig
Immunogen:
Synthetic peptides (N-terminal aa 1-17/16-36 of human MLDP)
Purification:
Stabilized antiserum
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:GP44
IHC analysis of human squamous cell carcinoma using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J.Hess
University Hospital Heidelberg).
IHC analysis of human skelatel muscle using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J.Hess
IHC analysis of human muscle using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J.Hess