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Antibodies

anti-Perilipin 2 (mouse N-term), guinea pig polyclonal antibody

Product Sizes
100 ul
£384.00
GP42-100UL
About this Product
SKU:
GP42
Additional Names:
Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Adipophilin / ADRP / PLIN2 (a member of the PLIN/PAT family) is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al.1998, 2013; Chong et al.). Polypeptide reacting: Specific for Adipophilin / ADRP / PLIN2, MW 46,646 (calculated from aa sequence data); apparent Mr 50,000 (after SDS-PAGE); pI 6.42. Tissue immunolocalization: Adipophilin / PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes; adipocytes are negative. Reactivity on cultured cell lines: 3T3-L1, OP9, HL-1 Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013). Chong, B. M. et al. The adipophilin C terminus is a self-folding membrane-binding domain that is important for milk lipid secretion. J. Biol. Chem. 286, 23254-23265 (2011). Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309-21 (1998).
Formulation:
Contains 0.09% sodium azide and 0.5% BSA
Host:
Guinea Pig
Immunogen:
Synthetic peptide (N-terminal aa 1-16 of murine adipophilin, also named PLIN2): MAAAVVDPQQSVVMRV-C
Purification:
Stabilized antiserum
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:GP42
Western blot analysis of undifferentiated 3T3-L1 and differentiated 3T3-L1 ALC (= adipocyte like cells) cell lysate with anti-Perilipin 2 antibody (Cat. No. GP42). Western blot analysis was performed on 30 ug of cell lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Perilipin 2 (Cat. No. GP42) was diluted in blocking buffer (1:500) and incubated at 4°C over-night. The secondary antibody goat anti-guinea pig HRP (Cat. No. 90001) was also diluted in blocking buffer (1:2
500) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
Immunofluorescence analysis of undifferentiated 3T3-L1 cells and differentiated 3T3-L1 ALC (= adipocyte like cells) cells with anti-Perilipin 2 antibody (Cat. No. GP42). The cells were differentiated using medium containing 0.5 mM IBMX
1 uM dexamethanosone and 10 ug/ml insulin. Fixation was performed using 3% paraformaldehyde for 15 min at RT. Cells were blocked with 5% BSA in PBST (PBS + 0.1% Tween 20) for 1 h at RT and permeabilized with 0.3% Triton-x 100 in PBS for 10 min at RT. The primary antibody anti-Perilipin 2 guinea pig polyclonal (Cat. No. GP42) was 1:100 diluted in blocking buffer and incubated over-night at 4°C. The secondary antibody donkey anti-guinea pig AF647 was also diluted in blocking buffer (antibody concentration 3.75 ug/ml) and incubated for 30 min at 37°C and 30 min at RT. DNA was stained with DAPI in blue.
Human skin (courtesy of J. Hess