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Antibodies

anti-Perilipin 3 N-terminus, guinea pig polyclonal antibody

Product Sizes
100 ul
£315.00
GP30-100UL
About this Product
SKU:
GP30
Additional Names:
Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17, PP17, PLIN3, M6PRBP1, TIP47
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
TIP47 (tail-interacting protein of 47 kDa; also named PLIN3) is involved in lipid droplet maturation. The protein has been localized in milk fat globule membranes of human and bovine origin. It has been described also as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. After radical surgery TIP47 serum levels are decreased (Than et al. 1999). TIP47 shows about 40% sequence homology to adipophilin (PLIN2). The GP30 antiserum, however, is specific for TIP47 (PLIN3) and does not cross-react with adipophilin (PLIN2) and perilipin (PLIN1) or other proteins of the PLIN/PAT family. Reactivity on cultured cell lines: PLC cells, pretreated with oleic acid. Than, N. G., Sumegi, B., Than, G. N., Kispal, G. & Bohn, H. Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): homology with adipophilin and the mouse adipose differentiation-related protein. Tumour Biol. J. Int. Soc. Oncodevelopmental Biol. Med. 20, 184-92 (1999).
Formulation:
Contains 0.09% sodium azide and 0.5% BSA
Host:
Guinea Pig
Immunogen:
Synthetic peptide of TIP47 N-terminus (aa 1-16)
Purification:
Stabilized antiserum
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:GP30
Mouse colon (courtesy of J.Hess
University Hospital Heidelberg)
Western blot analysis of HepG2 cell lysate with anti-Perilipin 3 antibody. Western blot analysis was performed on 30 ug HepG2 lysate and 39 ug HepG2 + OA lysate. Cells were previously treated with 400 uM oleic acid (OA) if indicated. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibodies anti-Perilipin 3 (N-terminus) guinea pig polyclonal (Cat. No. GP30) and anti-GAPDH were diluted in blocking buffer (1:500 anti-Perilipin
1.2 ug/ml anti-GAPDH
Cat. No. 690975) and incubated at 4°C over-night. The secondary antibodies donkey anti-guinea pig A647 and goat anti-mouse A488 were also diluted in blocking buffer (both 1:300) and incubated for 1 h at RT. The bands were visualized by fluorescent detection.