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Antibodies

anti-Keratin K13, guinea pig polyclonal antibody

Product Sizes
100 ul
£349.00
GP-K13-100UL
About this Product
SKU:
GP-K13
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Excellent marker to discriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those of different origin. Negative on foot sole, scalp. Reactive polypeptide: acidic human keratin K13 (Mr 55,000). Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngiom. Positive control: squamous epithelium of esophagus, gingival, palate, vagina (staining all of the suprabasal layers).
Formulation:
Contains 0.09% sodium azide and 0.5% BSA
Host:
Guinea Pig
Immunogen:
Synthetic peptide (TTTSSASV TTTSNA-C) of human keratin K13 (formerly also designated cytokeratin 13), coupled to KLH
Purification:
Stabilized antiserum
Reactivities:
Human, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:GP-K13
Human oesophagus (courtesy of L. Langbein)
Human oesophagus (courtesy of L. Langbein)
Human vagina (courtesy of L. Langbein)
Human tongue (courtesy of L. Langbein)
IHC analysis of human squamous epithelium of the buccal mucosa using anti-Keratin K13 (Cat. No. GP-K13). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Keratin K13 (Cat. No. GP-K13) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratries) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visible and with Haemalaun for a few minutes. The picture was acquired using microscopy (courtesy of J.Hess