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Antibodies

anti-Perilipin 2, AP125, mouse monoclonal antibody

Product Sizes
10 ug
£143.00
690102-10UG
50 ug
£384.00
690102-50UG
About this Product
SKU:
690102
Additional Names:
Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Application:
Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
AP125
Clonality:
Monoclonal
Concentration:
50 ug/ml
Extra Details:
Perilipin 2/Adipophilin/ADRP/PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir. Enhanced expression of Perilipin 2/Adipophilin/ADRP/PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study virus entry via lipid droplets. Polypeptide reacting: Perilipin 2/Adipophilin/ADRP/PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72. Immunolocalization: Perilipin 2/Adipophilin/ADRP/PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative.Also positively stained are lipid-storing CD 68-positive macrophages. Tested cultured cell lines: PLC, MDCK. Learn more about PROGEN Perilipin antibodies.
Formulation:
PBS buffer, pH 7.4 with 0.09% sodium azide and 0.5 % BSA
Host:
Mouse
Immunogen:
Synthetic peptide (aa 5-27 from N-terminus of human adipophilin/PLIN2)
Isotype:
IgG1
Purification:
Affinity Purified
Reactivities:
Bovine, Canine, Human, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:690102
Western blot analysis of HepG2 cell lysate with anti-Perilipin 2 antibody. Western blot analysis was performed on 15 ug HepG2 lysate and 15 ug HepG2 + OA lysate. Cells were previously treated with 400 uM oleic acid (OA) if indicated. Cells were lysed in RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Perilipin 2 (N-terminus) mouse monoclonal
AP125 (Cat. No. 690102) was diluted in blocking buffer (antibody concentration 0.5 ug/ml) and incubated at 4°C over-night. The secondary antibody goat anti-mouse IgG polyclonal
HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
IHC of human skin (courtesy of J.HeB Beta
University Hospital Heidelberg)