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Antibodies

anti-Cyclin D1, DCS-6, mouse monoclonal antibody

Product Sizes
20 ug
£124.00
690053-20UG
100 ug
£277.00
690053-100UG
About this Product
SKU:
690053
Additional Names:
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
DCS-6
Clonality:
Monoclonal
Concentration:
100 ug/ml
Extra Details:
DCS-6 reacts specifically with cyclin D1 protein present predominantly in G1 phase of cell cycle; it does not cross-react with D2 or D3 cyclins (the closest relatives within the cyclin family); DCS-6 is especially useful in tumor diagnosis: it can be used as fast method to identify cases with 11q13 amplifications. Cyclin D1 gene is identical to the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. Polypeptide reacting: Mr 36 kDa polypeptide (cyclin D1 polypeptide of human cells). Reactivity on cultured cell lines: MCF-7, MDA-MB-134 and MDA-MB-231 (breast cancer cell lines), UMSC22 (squamous cell carcinoma cell line), U-2-OS (osteosarcoma cell line), RT-112, A431, HT-29, 3T3-L1, Ins-1. The expression of Cyclin D1 is tissue specific and associated with tumor onset and progression.
Formulation:
0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4
Host:
Mouse
Immunogen:
Human recombinant full-length cyclin D1 protein
Isotype:
IgG2a
Purification:
Affinity Purified
Reactivities:
Canine, Human, Mouse, Non-human Primate, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:690053
Western blot analysis of mouse 3T3-L1 and rat Ins-1 cell lysate with anti-Cyclin D1 antibody. Western blot analysis was performed on 20 ug of either 3T3 or Ins-1 lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Cyclin D1 mouse monoclonal
DCS-6 (Cat. No. 690053) was diluted in blocking buffer (antibody concentration 2 ug/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal
HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
IHC analysis of human liver using anti-Cyclin D1. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Cyclin D1 mouse monoclonal