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Antibodies

anti-Keratin K19, Ks19.1, mouse monoclonal antibody

Product Sizes
5 ml
£207.00
65010-5ML
About this Product
SKU:
65010
Additional Names:
Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
Ks19.1|Ks19.1 (A53-B/A2)
Clonality:
Monoclonal
Extra Details:
Ks 19.1 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma. Polypeptide reacting: Mr 40,000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 311-335 (QSQLSMKAALEDTLAETEARFGAQL) of the alpha-helical fragment. Tumors specifically detected: all tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma. Reactivity on cultured cell lines: MCF-7.
Formulation:
PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
Host:
Mouse
Immunogen:
Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Isotype:
IgG2a
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:65010
IHC analysis of human liver using anti-Keratin K19 antibody (Cat. No. 61010). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Keratin K19 (Cat. No. 61010) was diluted in PBS (antibody concentration 1 ug/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The picture was acquired using microscopy (courtesy of J. Hess
University Hospital Heidelberg).
IHC analysis of human head and neck squamous cell carcinoma using anti-Keratin K19 antibody (Cat. No. 690010). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Keratin K19 (Cat. No. 690010) was diluted in PBS (antibody concentration 0.1 ug/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J. Hess
University Hospital Heidelberg).
IHC analysis of human colon using anti-Keratin K19 antibody (Cat. No. 61010). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Keratin K19 (Cat. No. 61010) was diluted in PBS (antibody concentration 10 ug/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The picture was acquired using microscopy (courtesy of J. Hess