anti-AAV VP1/VP2/VP3, B1, mouse monoclonal antibody, AFDyeTM 647
Product Sizes
50 ug
£574.00
61058-647-50UG
About this Product
- SKU:
- 61058-647
- Application:
- Dot Blot, Western Blot
- CE/IVD:
- RUO
- translate.label.attr.clone:
- B1
- Clonality:
- Monoclonal
- Conjugate:
- AFDye™ 647
- Concentration:
- 500 ug/ml
- Extra Details:
- The B1 antibody reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a reduced degree with assembled viral particles. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection. The AFDyeTM 647 is a bright, far-red-fluorescent dye ideally suited for the 633 nm or 647 nm laser lines (excitation/emission 648/671 nm). It is structurally similar to Alexa FluorR 647, and can be used with a common Cy5 filter set. A significant advantage to using long wavelength dyes such as Cy5 or AFDyeTM 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281-93 (2000).Alexa FluorR is a registered trademark of Thermo Fisher Scientific. Limited Use Label License: Research Use Only Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.
- Formulation:
- Liquid; PBS + 0.09% sodium azide
- Host:
- Mouse
- Immunogen:
- AAV2 capsids
- Isotype:
- IgG1
- Physical State:
- Liquid
- Purification:
- Affinity Purified
- Reactivities:
- Virus
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Progen
- Type:
- Antibody: Monoclonal Antibody
- Manufacturer's Data Sheet:61058-647
