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Antibodies

anti-Keratin K5/K8, C22, mouse monoclonal antibody

Product Sizes
50 ug
£303.00
61031-50UG
About this Product
SKU:
61031
Additional Names:
Keratin, type II cytoskeletal 74, Cytokeratin-74, CK-74, Keratin-5c, K5C, Keratin-74, K74, Type II inner root sheath-specific keratin-K6irs4, Type-II keratin Kb37, KRT74, K6IRS4, KB37, KRT5C, KRT6IRS4, Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
C22|Ks 5+8.22/C22
Clonality:
Monoclonal
Concentration:
50 ug/ml
Extra Details:
C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia. Polypeptide reacting: Mr 52,500, Mr 58,000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells. Epitope has been mapped to aa 353-367 on alpha helical rod domain of Keratin K8 (Waseem et al., 2004). Reactivity on cultured cell lines: MCF-7, RT 112, HT-29, HaCaT, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK. Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004).
Formulation:
Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Host:
Mouse
Immunogen:
Human keratin K8, purified from SDS PAGE gel
Isotype:
IgG1
Physical State:
Lyophilized
Purification:
Affinity Purified
Reactivities:
Bovine, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C reconstituted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Progen
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:61031
Western blot analysis of human HaCaT lysate with anti-Keratin K5/K8 antibody. Western blot analysis was performed on 10 ug HuCaT lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Keratin K5/K8 mouse monoclonal
C22 (Cat. No. 690031) was diluted in blocking buffer (antibody concentration 0.1 ug/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal
HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
IHC of mouse tongue (courtesy of J.HeB Beta
University Hospital Heidelberg)