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Antibodies

anti-Keratin K19, Ks19.2, mouse monoclonal antibody

Product Sizes
50 ug
£300.00
61029-50UG
About this Product
SKU:
61029
Additional Names:
Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
Ks19.2|Ks19.2 (Z105.6)(also published as BM 19.21, MAK 19.21)
Clonality:
Monoclonal
Concentration:
50 ug/ml
Extra Details:
Ks 19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma! Polypeptide Reacting: Mr 40,000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the alpha-helical fragment. Tumors specifically detected: all tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma. Reactivity on cultured cell lines: MCF-7.
Formulation:
Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5 % BSA in PBS buffer, pH 7.4)
Host:
Mouse
Immunogen:
Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Isotype:
IgG2b
Physical State:
Lyophilized
Purification:
Affinity Purified
Reactivities:
Amphibian, Avian, Bovine, Human, Mouse, Rabbit, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C reconstituted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Progen
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:61029
IHC of human oral mucosa (courtesy of J.HeB Beta
University Hospital Heidelberg)
Western blot analysis of human MCF-7 cell lysate with anti-Keratin K19 antibody. Western blot analysis was performed on 10 ug or 5 ug of MCF-7 lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Keratin K19 mouse monoclonal
Ks19.2 (Z105.6; Cat. No. 690029) was diluted in blocking buffer (antibody concentration 0.1 ug/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal
HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.