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Antibodies

anti-Vimentin, guinea pig polyclonal antibody

Product Sizes
50 ug
£371.00
610130-50UG
About this Product
SKU:
610130
Additional Names:
Vimentin, VIM
Application:
ELISA, Immunohistochemistry, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
50 ug/ml
Extra Details:
Specific detection of vimentin (57 kDa polypeptide). Tumors specifically detected: Sarcoma (including myosarcoma), lymphoma, melanoma.
Formulation:
Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Host:
Guinea Pig
Immunogen:
Human Vimentin
Physical State:
Lyophilized
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C reconstituted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Progen
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:610130
Western blot analysis of HeLa lysate with anti-Vimentin antibody. Western blot analysis was performed on 10 ug wild type (WT) and 10 ug Vimentin knockout (KO) HeLa lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Vimentin guinea pig polyclonal (Cat. No. 610130) was diluted in blocking buffer (antibody concentration 0.02 ug/ml) and incubated for 1 h at RT. The secondary antibody anti-guninea pig
HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
Western blot analysis of human HeLa and mouse C2C12 lysate with anti-Vimentin antibody. Western blot analysis was performed on 20 ug HeLa and 18 ug C2C12 lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Vimentin guinea pig polyclonal (Cat. No. 610130) was diluted in blocking buffer (antibody concentration 0.02 ug/ml) and incubated for 1 h at RT. The secondary antibody anti-guinea pig
HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
IHC analysis of murine kidney using anti-Vimentin antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Vimentin guinea pig polyclonal (Cat. No. 610130) was diluted in PBS (antibody concentration 1 ug/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes (courtesy of J. Hess