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Antibodies

anti-Vimentin, VIM3B4, mouse monoclonal antibody

Product Sizes
50 ug
£317.00
61013-50UG
About this Product
SKU:
61013
Additional Names:
Vimentin, VIM
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
VIM 3B4|VIM3B4
Clonality:
Monoclonal
Concentration:
50 ug/ml
Extra Details:
The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin. Polypeptide reacting: 57 kDa intermediate filament protein (vimentin) of mesenchymal cells. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Tested cultured cell lines: fibroblasts (SV-80). Bohn W, Wiegers W, Beuttenmueller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992). Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).
Formulation:
Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Host:
Mouse
Immunogen:
Vimentin purified from bovine lens
Isotype:
IgG2a, kappa
Physical State:
Lyophilized
Purification:
Affinity Purified
Reactivities:
Amphibian, Avian, Bovine, Human, Mouse, Non-human Primate
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C reconstituted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Progen
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:61013
Western blot analysis of HeLa lysate with anti-Vimentin antibody. Western blot analysis was performed on 10 ug wild type (WT) and 10 ug Vimentin knockout (KO) HeLa lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Vimentin mouse monoclonal
VIM 3B4 (Cat. No. 690013) was diluted in blocking buffer (antibody concentration 33 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG
HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
WB with anti-Vimentin antibody (Cat. No. 61013
1:500)