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Antibodies

anti-Keratin K13, Ks13.1, mouse monoclonal antibody

Product Sizes
50 ug
£275.00
61007-50UG
About this Product
SKU:
61007
Additional Names:
Cytokeratin 13
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
Ks13.1
Clonality:
Monoclonal
Concentration:
50 ug/ml
Extra Details:
Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting: Mr 54,000 polypeptide human keratin K13 (formerly designated cytokeratin 13; with minor affinity to keratins K14, Mr 50,000, and K16, Mr 48,000 and also to K25, formerly designated K25irs1 from inner root sheath of hair follicle). Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma. Reactivity on cultured cell lines: cell lines from squamous cell CA, e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder CA.
Formulation:
Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Host:
Mouse
Immunogen:
Keratin K13 of Mr 54,000 purified from human esophagus
Isotype:
IgG1
Physical State:
Lyophilized
Purification:
Affinity Purified
Reactivities:
Bovine, Human, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C reconstituted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Progen
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:61007
Mouse tongue (courtesy of L. Langbein)
IHC analysis of rat tongue using anti-Keratin K13 (Cat. No. 61007). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Keratin K13 (Cat. No. 61007) was diluted in PBS (1:6
8 ug/ml) and incubated at 4°C over-night. The secondary antibody biotin anti-mouse was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratries) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The picture was acquired using microscopy (courtesy of J.Hess
University Hospital Heidelberg).
WB with anti-Keratin K13 antibody (Cat. No. 61007