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Antibodies

anti-mTOR, rabbit, polyclonal antibody

Product Sizes
100 ul
£275.00
490947-100UL
About this Product
SKU:
490947
Additional Names:
Mechanistic target of rapamycin, Mammalian target of rapamycin
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
500 ug/ml
Extra Details:
Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in protein synthesis, cell growth, proliferation and differentiation. It is inhibited by rapamycin, which has immunosuppressant functions. The 290 kDa protein is highly regulated by growth factors and nutrients like glucose and amino acids. mTOR is involved in several pathways from protein degradation via autophagy to protein synthesis via translation initiation (4EBP), ribosome and lipid synthesis. It is located in vesicles and the cytosol.
Formulation:
PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA
Host:
Rabbit
Immunogen:
Peptide from human mTOR (GTTVPESIHSFIGDGLVKPE)
Purification:
Affinity Purified
Reactivities:
Canine, Human, Mouse, Non-human Primate, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:490947
Immunocytochemistry (ICC) analysis of HeLa Cl6 cells with anti-mTOR antibody (Cat. No. 490947). HeLa cells were blocked with 5% BSA in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-mTOR rabbit polyclonal was diluted in blocking buffer (antibody concentration 1 ug/ml) and incubated over-night at 4°C. The secondary antibody goat anti-rabbit Alexa Fluor 488 conjugate was also diluted in blocking buffer (antibody concentration 2.5 ug/ml) and incubated for 30 min at 37°C and 30 min at RT. DNA was stained with DAPI in blue.
Western blot analysis of HEK and HeLa lysate with anti-mTOR antibody (Cat. No. 490947). Western blot analysis was performed on 50 ug HeLa lysate and 40 ug HEK lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1.5 h at RT. The primary antibody anti-mTOR rabbit polyclonal was diluted in blocking buffer (antibody concentration 250 ng/ml) and incubated over-night at 4°C. The secondary antibody goat anti-rabbit IgG HRP (Cat. No. 90003) was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
Western blot analysis of HEK lysate with anti-mTOR antibody (Cat. No. 490947). Western blot analysis was performed on 20 ug wild type (WT) HEK lysate and 20 ug mTOR knock-out (KO) HEK lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-mTOR rabbit polyclonal was diluted in blocking buffer (antibody concentration 500 ng/ml) and incubated for 1 h at RT. The secondary antibody goat anti-rabbit IgG HRP (Cat. No. 90003) was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
Immunohistochemistry (IHC) analysis of human head and neck squamous cell carcinoma using anti-mTOR antibody (Cat. No. 490947). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-mTOR