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Antibodies

Mouse Anti-Beta actin Antibody [B4-B2], Mouse monoclonal Antibody

Product Sizes
100uL
M1210-2-M1210-2
About this Product
SKU:
M1210-2
Application:
WB, IF-Cell, IHC-Paraffin, FC
Buffer:
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
B4-B2
Clonality:
Mouse monoclonal Antibody
Concentration:
2 mg/mL.
Control:
NIH/3T3, NIH/3T3 cell lysate, PC12 cell lysate, MCF-7 cell lysate, HepG2 cell lysate, Hela cell lysa
Format:
Liquid
Host:
Mouse
Immunogen:
Synthetic peptide within N-terminal of Human beta actin.
Isotype:
IgG1
Molecular Weight:
Predicted band size: 42 kDa
Purification:
Immunogen affinity purified.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20℃. Stable for 12 months from date of receipt.
Supplier:
Huabio
ICC staining of Beta actin in NIH/3T3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (M1210-2, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Beta actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1210-2, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: PC-12 cell lysate Lane 3: MCF-7 cell lysate Lane 4: HepG2 cell lysate Lane 5: Hela cell lysate Lane 6: Mouse lung tissue lysate
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta actin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta actin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.