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Antibodies

Rabbit Anti-E2F6 Antibody [JE33-69], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
HA721481-HA721481
About this Product
SKU:
HA721481
Application:
WB, IHC-Paraffin
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE33-69
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
Jurkat cell lysate, K-562 cell lysate, mouse brain tissue.
Format:
Liquid
Host:
Rabbit
Immunogen:
Recombinant protein within Human E2F6 aa 182-281 / 281.
Isotype:
IgG
Molecular Weight:
Predicted band size: 32 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of E2F6 on different lysates with Rabbit anti-E2F6 antibody (HA721481) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721481) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-E2F6 antibody (HA721481) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721481) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.