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Antibodies

Mouse Anti-SALL4 Antibody [A9G9-R], Recombinant Mouse monoclonal Antibody

Product Sizes
100uL
HA601168-HA601168
About this Product
SKU:
HA601168
Application:
WB, IHC-Paraffin, IF-Tissue, IF-Cell, FC
Buffer:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A9G9-R
Clonality:
Recombinant Mouse monoclonal Antibody
Concentration:
1 mg/mL.
Control:
NCCIT cell lysates, human seminoma tissue, human embryonal carcinoma tissue, human testis tissue, NC
Format:
Liquid
Host:
Mouse
Immunogen:
Recombinant protein within human SALL4 aa 904-1,053/1,053.
Isotype:
IgG1
Molecular Weight:
Predicted band size: 112 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of SALL4 on NCCIT cell lysates with Mouse anti-SALL4 antibody (HA601168) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 112 kDa Observed band size: 140/110/75 kDa Exposure time: 1 minute 26 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601168) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Mouse anti-SALL4 antibody (HA601168) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601168) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue with Mouse anti-SALL4 antibody (HA601168) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601168) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-SALL4 antibody (HA601168) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601168) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative control) with Mouse anti-SALL4 antibody (HA601168) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601168) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.