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Antibodies

Rabbit Anti-Phospho-HER3/ErbB3 (Y1222) Antibody [JE51-89], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
ET7110-10-ET7110-10
About this Product
SKU:
ET7110-10
Application:
WB, IHC-Paraffin
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE51-89
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL
Control:
Human thyroid tissue, human skin tissue, human placenta tissue.
Format:
Liquid
Host:
Rabbit
Immunogen:
Synthetic phosphopeptide corresponding to a region surrounding Tyrosine 1222 of Human ErbB3.
Isotype:
IgG
Molecular Weight:
Predicted band size: 148 kDa.
Purification:
Protein A affinity purified.
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HER3/ErbB3 Phospho (pY1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-HER3/ErbB3 Phospho (pY1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HER3/ErbB3 Phospho (pY1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.