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Antibodies

Rabbit Anti-CBFb Antibody [JG39-35], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
ET7108-87-ET7108-87
About this Product
SKU:
ET7108-87
Application:
WB, IHC-Paraffin, FC
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JG39-35
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
Rat kidney tissue, human kidney tissue, SiHa, mouse lung tissue, mouse hippocampus tissue.
Format:
Liquid
Host:
Rabbit
Immunogen:
Recombinant protein within Human CBFb aa 60-182 / 182.
Isotype:
IgG
Molecular Weight:
Predicted band size: 22 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CBFb antibody (ET7108-87) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-87) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.
FlowCytometric analysis of SiHa cells with CBFb antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-CBFb antibody (ET7108-87) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-87) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CBFb antibody (ET7108-87) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-87) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.