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Antibodies

Rabbit Anti-GAPDH Antibody [SA30-01], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
ET1601-4-ET1601-4
About this Product
SKU:
ET1601-4
Application:
WB, IF-Cell, IF-Tissue, IHC-Paraffin, FC, IP
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SA30-01
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
Rat liver tissue lysate, rat lung tissue lysate, rat heart tissue lysate, rat cerebellum tissue lysa
Format:
Liquid
Host:
Rabbit
Immunogen:
Recombinant protein within mouse GAPDH aa 94-333 / 333.
Isotype:
IgG
Molecular Weight:
Predicted band size: 36 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat, Chicken, Zebrafish
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of GAPDH on Hela cell lysates with Rabbit anti-GAPDH antibody (ET1601-4). Hela cell lysates at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4, 1/5,000) was used in 5% NFDM/TBST at room temperature for 1 hour. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 45 mins at room temperature. Positive control: Lane 1: PC-3 cell lysate, 10 µg/Lane Lane 2: Mouse colon tissue lysate, 20 µg/Lane Lane 3: SH-SY5Y cell lysate, 10 µg/Lane Lane 4: PC-3 cell lysate, 10 µg/Lane Lane 5: NIH/3T3 cell lysate, 10 µg/Lane Lane 6: SK-Br-3 cell lysate, 10 µg/Lane Lane 7: Rat brain tissue lysate, 20 µg/Lane
Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4, 1/10,000) was used in 5% NFDM/TBST at room temperature for 1 hour. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 45 mins at room temperature. Positive control: Lane 1: Rat liver tissue lysate, 20 µg/Lane Lane 2: Rat lung tissue lysate, 20 µg/Lane Lane 3: Rat lung tissue lysate, 20 µg/Lane Lane 4: Rat heart tissue lysate, 20 µg/Lane Lane 5: Rat cerebellum tissue lysate, 20 µg/Lane Lane 6: Rat skeletal muscle tissue lysate, 20 µg/Lane Lane 7: Rat spleen tissue lysate, 20 µg/Lane Lane 8: Rat small intestine tissue lysate, 20 µg/Lane
Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (ET1601-4) at 1/80,000 dilution. Cell lysates at 10 µg/Lane, tissue lysates at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4) at 1/80,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GAPDH on DF-1 cell lysates with Rabbit anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Cell lysates at 15 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 second; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 2 hour at room temperature. The primary antibody (ET1601-4) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 ℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 2 hour at room temperature.