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Antibodies

Rabbit Anti-VCAM1 Antibody [SA05-04], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
ET1601-18-ET1601-18
About this Product
SKU:
ET1601-18
Application:
WB, IF-Cell, IF-Tissue, IHC-Paraffin, IP
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SA05-04
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
C2C12 cell lysate, 3T3-L1 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, NIH/3T3
Format:
Liquid
Host:
Rabbit
Immunogen:
Synthetic peptide within Human VCAM1 aa 690-739 / 739.
Isotype:
IgG
Molecular Weight:
Predicted band size: 81 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat, Zebrafish
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of VCAM1 on different lysates with Rabbit anti-VCAM1 antibody (ET1601-18) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate (20 µg/Lane) Lane 2: 3T3-L1 cell lysate (20 µg/Lane) Lane 3: Mouse spleen tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 81 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-18) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VCAM1 on different lysates with Rabbit anti-VCAM1 antibody (ET1601-18) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (negative) (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 81 kDa Observed band size: 100 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-18) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
ICC staining of VCAM1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VCAM1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-18, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-VCAM1 antibody (ET1601-18) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-18) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.