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Antibodies

Rabbit Anti-Integrin beta 1 Antibody [SA40-08], Recombinant Rabbit monoclonal Antibody [NeuroAb]

Product Sizes
100uL
ET1601-17-ET1601-17
About this Product
SKU:
ET1601-17
Application:
WB, IHC-Paraffin
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SA40-08
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
HeLa cell lysate, U-87 MG cell lysate, A431 cell lysate, Neuro-2a cell lysate, C6 cell lysate, human
Format:
Liquid
Host:
Rabbit
Immunogen:
Recombinant protein within Human Integrin beta 1 aa 1-240 / 798.
Isotype:
IgG
Molecular Weight:
Predicted band size: 88 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat, Zebrafish
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Integrin beta 1 on different lysates with Rabbit anti-Integrin beta 1 antibody (ET1601-17) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 88 kDa Observed band size: 120-140 kDa Exposure time: 1 minutes 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-17) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Integrin beta 1 antibody (ET1601-17) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-17) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Integrin beta 1 antibody (ET1601-17) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-17) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Integrin beta 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-17, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Integrin beta 1 antibody (ET1601-17) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-17) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.