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Antibodies

Anti-RCAN1/Dscr1 (central region) Antibody

Product Sizes
100 ul
RP3961-100UL
About this Product
SKU:
RP3961
Additional Names:
Dscr1, MCIP, RCAN1, calcipressin, Adapt78
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our RCAN1/Dscr1 (central region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat RCAN1/Dscr1 (central region) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
RCAN1
Host:
Rabbit
Immunogen:
RCAN1 synthetic peptide (coupled to carrier protein) corresponds to amino acids from the central region of human RCAN1S (isoform 2). This sequence is highly conserved in rat and mouse RCAN1, and this sequence is found in all four isoforms of RCAN1. In addition, this sequence has significant homology to RCAN2 and RCAN3.
Isotype:
IgG
Molecular Weight:
38
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects several isoforms of RCAN1 on SDS-PAGE immunoblots of mouse C2C12, rat PC12, and human Jurkat cells. The predominate isoform detected is the 38 kDa RCAN1L, but the antibody may also detect RCAN2 and RCAN3.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Western blot analysis of RCAN1 expression in human Jurkat (lanes 1 & 4), mouse C2C12 (lanes 2 & 5), and rat PC12 (lanes 3 & 6). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000 and anti-RCAN1 (a.a. 132-140) at 1:500.
Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (RP3941) and anti-RCAN1 (a.a. 132-140) (RP3961) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide RX3945 and RX3965, respectively. The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.