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Antibodies

anti-Enolase 1 antibody

Product Sizes
100 ul
£593.00
ARG55159-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG55159
Additional Names:
MPB1; Plasminogen-binding protein; Alpha-enolase; MBP-1; NNE; PPH; Enolase 1; ENO1L1; Phosphopyruvate hydratase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Non-neural enolase; MPB-1; EC 4.2.1.11
Application:
Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS and 0.09% (W/V) Sodium azide
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
Host:
Rabbit
Isotype:
IgG
Purification:
SAS Precipitated; Dialysis
Reactivities:
Amphibian, Avian, Bovine, Non-human Primate, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Flow Cytometry: HeLa cells stained with ARG55159 anti-Enolase 1 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
Immunofluorescence: C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG55159 anti-Enolase 1 antibody (green) at 1:25 dilution, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lymph stained with ARG55159 anti-Enolase 1 antibody.
Western blot: 30 ug of HeLa, Jurkat , HepG2, DU145, MDAMB231, HEK293T, Mouse heart, Mouse liver, Rat heart and Rat liver lysates stained with ARG55159 anti-Enolase 1 antibody at 1:500 dilution.