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Cellecta

Cellecta Inc. provides custom and contract solutions for high-throughput genetic screening needs. Their research organisation focuses primarily on developing and implementing flexible and scalable broad-based screening and analysis approaches that identify the mechanisms regulating biological processes to expedite the discovery and characterisation of novel therapeutic targets and drugs. The unique capabilities of lentiviral and shRNA technologies provide a platform that enables genome-wide functional screening and the identification and validation of genes involved in critical biological and pathological responses.

Cellecta

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CELLECTA CRISPR Brochure 

 

Constructs

 

Achieve complete knockout, or knock-in or knock- down your gene with our reliable and precise CRISPR lentiviral constructs, available as plasmids or packaged, transduction-ready lentiviral particles. Pick your gene of interest, choose your options, and we do the rest.

  • Effective guide design increases efficiency
  • CRISPR KO, CRISPRi and CRISPRa constructs available
  • Choice of single vector (both sgRNA & Cas9 in one vector) or two vector system (sgRNA & Cas9 in separate vectors)

          

 

Custom CRISPR Construct Options

Construct Choices

Benefits

Customizable Options

Single Vector

CRISPR/Cas9

System

•Convenient single transduction for knockout

•Ideal for in vivo

knockout experiments

•Choice of antibiotic selection or fluorescent marker (PuroR, HygroR, BlastR, NeoR, RFP, or GFP)

Two Vector CRISPR/Cas9

System

•Better knockout efficiency

•Significantly higher titers

•Ideal for CRISPR libraries/screening applications

•Constitutive or inducible sgRNA expression

•Choice of antibiotic selection and fluorescent marker (PuroR, HygroR, BlastR, NeoR, and RFP / GFP)

 

Libraries

 

Pooled lentiviral-based libraries containing heterogeneous mixtures of CRISPR single-guide RNA (sgRNA or gRNA) constructs allow you to assay the effects of many thousands of knockouts in one experiment. As we have done for over 10 years with pooled shRNA libraries, Cellecta has the expertise and capability to generate high-quality, complex CRISPR sgRNA libraries targeting virtually any defined sequences.

 

CRISPR Genome-Wide Knockout, CRISPRa and CRISPRi Ready-to-Use Libraries

CRISPR Genome-wide libraries are available for human and mouse in both plasmid and ready-to-use packaged formats.

  • Superior quality, highly representative targeted lentiviral sgRNA for CRISPR knockout, activation or inhibition screens
  • Off-the-shelf lentiviral-based CRISPR sgRNA libraries
  • Target nearly all protein-encoding genes in the human or mouse genome
  • Improved sgRNA designs increase knockout, knock-in or knockdown effectiveness

 

Custom CRISPR Library Generation

You give us your list of targets, and we will do the rest! We design sgRNA, generate oligos, clone, and sequence the library using NGS. Our sgRNA design includes parameters that optimize for on-target knockout while minimizing off-target cross reactivity. We can also design sgRNA for CRISPRi and CRISPRa screens. Libraries include non-targeting, intron-targeting, and lethal sgRNA controls to provide reference standards when analyzing screening results

 

Loss-of-Function Screening Services

Cellecta has developed an effective, scalable, high-throughput CRISPR screening services using the cell model of your choice. Choose from a variety of custom and contract solutions for high-throughput genetic screening needs.

Dropout Viability Screening

  • Identify genes essential for growth and proliferation
  • Find targets that increase cell sensitivity to compounds or treatments
  • Look for synthetically lethal interactions with known genetic lesions

sgRNA Rescue Screening

  • Identify genes functionally required for cell sensitivity to a treatment or compoundIdentify genes functionally required for cell sensitivity to a treatment or compound
  • Identify pathway or gene clusters mediating a specific cell response
  • Elucidate the mechanism of action or target pathway for a compound or drug

 

Knockout cells

 

Choose your gene of interest and cell background, and Cellecta will build a custom knockout cell line.

 

  • Knockout validated by sequence verification
  • Transient use of Cas9 to make knockout
  • Transient sgRNA "zero-footprint" protocol available

 

CRISPRtest Cas9 Assay Kits

 

Want to know if Cas9 is active in your cells? Find out with Cellecta’s CRISPRtest Functional Cas9 Activity Kit. We also offer test kits to measure

  • dCas9-Activator enhancement
  • dCas9-Repressor function

A standard FACS analysis detects Cas9 activity in your cells.

 

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