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Antibodies

Kir2.1 Antibody, Clone S112: PerCP

Product Sizes
100 ug
SMC-310D-PCP-100UG
About this Product
SKU:
SMC-310D-PCP
Additional Names:
HHBIRK1 Antibody, HHIRK1 Antibody, HIRK 1 Antibody, IRK1 Antibody, KCNJ2 Antibody, LQT7 Antibody, SQT3 Antibody, potassium inwardly rectifying channel J2 Antibody
Application:
Antibody Microarray, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
S112
Clonality:
Monoclonal
Conjugate:
PerCP
Concentration:
1 mg/ml
Extra Details:
The Kir2.1 inward-rectifier potassium ion channel is encoded by the KCNJ2 gene. A defect in this gene is associated with Andersen-Tawil syndrome (1).
Host:
Mouse
Immunogen:
Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Non-human Primate, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~45kDa. No cross-reactivity against Kir2.2 or Kir2.3.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir2.1 Monoclonal Antibody, Clone S112 (SMC-310). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir2.1 Monoclonal Antibody (SMC-310) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (SMC-310). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (SMC-310) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.
Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (SMC-310). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (SMC-310) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (SMC-310). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (SMC-310) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.