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Antibodies

PSD 95 Antibody, Clone 6G6: ATTO 488

Product Sizes
100 ug
SMC-122D-A488-100UG
About this Product
SKU:
SMC-122D-A488
Additional Names:
PSD 95 Antibody, PSD-95 Antibody, DLG4 Antibody, SAP90 Antibody, Synapse-associated protein 90 Antibody, Postsynaptic density protein 95 Antibody, Disks large homolog 4 Antibody
Application:
Antibody Microarray, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
6G6
Clonality:
Monoclonal
Conjugate:
ATTO 488
Concentration:
1 mg/ml
Extra Details:
Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).
Host:
Mouse
Immunogen:
Recombinant rat PSD-95
Isotype:
IgG2a
Purification:
Protein A Purified
Reactivities:
Bovine, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~100kDa. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6 (SMC-122). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (SMC-122) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Junction staining.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6 (SMC-122). Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (SMC-122) at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.
Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6 (SMC-122). Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody (SMC-122) at 1:1000.