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Biomaterials

Guinea Pig Complement (Fresh Frozen)

Product Sizes
100 ml
C300-0100-100ML
About this Product
SKU:
C300-0100
Additional Names:
Complement system, tissue macrophages, blood monocytes, protease C3-convertase, mannose-binding lectin pathway, C3, C3a, C3b, C5a, C5b, C6, C7, C8, and polymeric C9, cascade cleavage and activation events, recruit inflammatory cells, anaphylatoxin
Application:
Cell-based/Functional Assay, ELISA, Flow Cytometry, Purification
Buffer:
None
CE/IVD:
RUO
Concentration:
85 mg/ml
Extra Details:
Special processing techniques are used to yield products with high complement activity and low background cytotoxicity. Guinea Pig Complement is suitable for CFT and SRH.
Physical State:
Frozen
Shipping Conditions:
Dry Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Biomaterials: Blood Products
Individual serum Inaba vibriocidal responses for each of the four dosages (107, 108, 109, or 1010 CFU) or placebo are indicated. Within each dosage group, the five circles denote (from left to right) the following five time points: baseline and 7, 10, 14, and 28 days postvaccination. A closed circle indicates the peak response for an individual. The vibriocidal antibody assay compares the amount of V. cholerae growth achieved in a 96-well plate when mixed with guinea pig complement (p/n C300-0050) of a standard activity and serial dilutions of the heat-inactivated human serum samples, all assayed in duplicate. FIG 1. PMID: 25410205.
Ccr2-KO mice exhibit enhanced BCR proximal signalling. B cells were purified from splenic mononuclear cells by incubation of anti-Thy-1 and guinea pig complement (p/n C300-0500) for 30 min. Purified splenic B cells were incubated with AF546-F(ab')2-anti-mouse-Ig (M + G) at 4°C for 30 min and activated at 37°C for 5, 10 and 30 min, confocal microscopy (CFm) was performed. Cells were incubated with biotin-conjugated F(ab')2-anti-mouse-Ig (M + G) and streptavidin, then activated at 37°C for 5, 10 and 30 min, western blotting was performed. (A) Representative CFm images of phosphorylated CD19 (pCD19) and BCR (60x objective, scale bar = 2.5 um). (B) Colocalization between pCD19 and BCR. (C) Western blotting of pCD19, pSyk, pCD79a expression in B cells. (D) Representative CFm images of pY and BCR (60x objective, scale bar = 2.5 um). (E) Colocalization between pY and BCR. (F) Colocalization between pBTK and BCR. (G) Western blotting of pY expression in B cells. (H) Western blotting of pBTK and BTK expression in B cells. (I) Colocalisation between pSHIP-1 and BCR. (J) Western blotting of pSHIP-1 and SHIP-1 expression in B cells. (K) Western blotting of pBTK and pSHIP-1 in WT B cells, Ccr2-KO B cells and Ccr2-KO B cells treated with 5uM R406. (L) Representative image of intracellular Ca2+ flux kinetics in WT and Ccr2-KO B cells following stimulation with 10 ug/ml biotin-conjugated F(ab')2 anti-mouse Ig (M + G). All images were representative images from three independent experiments. The number of cells analyzed for each parameter in CFm assay was 30-50. Error bars were shown as mean (+/- SD). *p < .05, **p < .01, ***p < .001, ns: no statistical significance. Fig 2. PMID: 35875970.
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