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Antibodies

PINK1 truncated Antibody

Product Sizes
25 ul
600-401-GU5S-25UL
About this Product
SKU:
600-401-GU5S
Additional Names:
PINK1|rabbit anti-PINK1 antibody, Serine/threonine-protein kinase PINK1 (mitochondrial), BRPK, PTEN-induced putative kinase protein 1, PTEN-induced kinase
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.02 mg/ml
Extra Details:
PINK1 (PTEN induced putative kinase 1) belongs to the protein kinase superfamily and Ser/Thr protein kinase family. PINK1 protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. It is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at Ser-65 of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form. PINK1 interacts with PARK2 and FBXO7 and forms a complexes with PARK2 and PARK7. PINK1 is associated with Parkinson disease 6 (PARK6). Anti-PINK1 Antibody is useful for researcher interested in neurological research, neurodegenerative disorders, Pink/Parkin Mediated Mitophagy, and Parkinsons Disease Pathway.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-PINK1 truncated antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human PINK1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human PINK1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and 93% identity to mouse.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of Rabbit anti-PINK1 truncated antibody. Cell line: NIH 3T3 (p/n W10-000-358). Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PINK1 truncated antibody at 20 ug/mL for overnight at 4°C. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 5 ug/ml for 2 hrs at RT. Localization: PINK1 is mitochondrial and cytoplasmic. Staining: PINK1 as green fluorescent signal with DAPI (blue) nuclear counterstain.
Western Blot of Rabbit anti-PINK1 truncated antibody. Lane 1: recombinant PINK1. Load: 1 ug per lane. Primary antibody: PINK1 truncated antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody p/n (611-103-122) at 1:70,000 for 45 min at RT. Block: Odyssey blocking buffer overnight at 4°C. Predicted/Observed size: ~62, 30/~30 kda for PINK1. Other band(s): PINK1 phosphorylates and experiences rapid degradation causing various bands on WB.
Western Blot of Rabbit anti-PINK1 truncated antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: HEK293T untransfected lysate (W09-001-GX5). Lane 3: PINK1-overexpressing 293T. Load: 10 ug per lane. Primary antibody: PINK1 truncated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti-RABBIT IgG (H&L) (DONKEY) Antibody DyLight™ 488 Conjugated p/n (611-741-127) at 1:70,000 for 45 min at RT. Block: Odyssey blocking buffer overnight at 4°C. Predicted/Observed size: ~62, 30/~30 kda for PINK1.