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Antibodies

CIDE-B Antibody

Product Sizes
100 ug
600-401-AK7-100UG
About this Product
SKU:
600-401-AK7
Additional Names:
CIDE-B Antibody, CIDE-B, AI790179, 1110030C18Rik, Cell death activator CIDE-B, Cell death-inducing DFFA-like effector B|CIDEB
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes and bone marrow.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CIDE-B antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 14 amino acids near the internal region of human CIDE-B.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-CIDE-B Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. CIDE-B antibody has no cross activity to CIDE-A.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of CIDE-B antibody. Tissue: Human small intestine cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: CIDE-B antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CIDE-B is localized in the cytosol, lipid particles, and perinucear region of the cytoplasm. Staining: CIDE-B as red fluorescent signal.
Immunohistochemistry of CIDE-B antibody. Tissue: Human small intestine tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: CIDE-B antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: CIDE-B is localized in the cytosol, lipid particles, and perinuclear region of the cytoplasm. Staining: CIDE-B as precipitated brown signal with hematoxylin purple nuclear counterstain.
Western Blot of CIDE-B antibody. Lane 1: Mouse small intestine tissue lysate with CIDE-B at 0.5 ug/mL. Lane 2: Mouse small intestine tissue lysate with CIDE-B at 1 ug/mL. Lane 3: Mouse small intestine tissue lysate with CIDE-B at 2 ug/mL. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 24.8 kDa, 35 kDa for CIDE-B.